Application
Western blot analysis of extract from 293 cells using hnRNP A1 (Ab-192).| Product Name | hnRNP A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, hnRNPA1, hnRNP-A1, hnRNP A1, MGC102835 |
| Product description | Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism and transport of nuclear pre-mRNA transcripts . hnRNP A1 regulates the alternative splicing of c-Src, c-H-Ras and modifies initiation of translation of the fibroblast growth factor 2 mRNA. hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells . Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus . hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production . HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm ,1) Myer, V. E. and Steitz, J. A. (1995) RNA 1, 171-82. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53. Patry, C. et al. (2003) Cancer Res 63, 7679-88. Pinol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2. Siomi, M. C. et al. (1997) J Cell Biol 138, 1181-92. Black, A. C. et al. (1996) Virus Genes 12, 275-85. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91. Monette, A. et al. (2009) J Biol Chem 284, 31350-62. |
| Immunogen | hnRNP A1 (Ab-192) antibody was raised against a peptide sequence around aa.190~194 (S-S-S-Q-R) derived from Human hnRNP A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPA1 |
|---|---|
| Alternative Names | ALS19 ALS20 HNRPA1 IBMPFD3 HNRPA1L3 hnRNPA1 hnRNP-A1 hnRNP A1 MGC102835 |
| Molecular Weight(MW) | 34, 39 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total hnRNP A1 protein. |
| Entrez Gene | 3178 |
|---|---|
| Protein Accession | NP_002127.1 |
Application
Western blot analysis of extract from 293 cells using hnRNP A1 (Ab-192).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
