Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate).Application
Antibody used in IF on MCF7 cells at 1:200.Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPL ppt. k562 sample ).Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | HNRPL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRPL, HNRPL, hnRNP-L, P/OKcl.14 |
| Product description | Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.1) Guang, S., (2005) Mol. Cell. Biol. 25 (15), 6303-6313. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HNRPL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| HNRPL antibody can be used for detection of HNRPL by ELISA at 1:62500. HNRPL antibody can be used for detection of HNRPL by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPL |
|---|---|
| Alternative Names | HNRPL HNRPL hnRNP-L P/OKcl.14 |
| Molecular Weight(MW) | 65 kDa |
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Application
Antibody used in WB on HeLa S3, MCF7, K562 at 1:4000 (Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate).
Application
Antibody used in IF on MCF7 cells at 1:200.
Application
Antibody used in IP on K562 at 1:4000 (Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPL ppt. k562 sample ).
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HNRPL antibody can be used for detection of HNRPL by ELISA at 1:62500. HNRPL antibody can be used for detection of HNRPL by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HNRPL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
