Application
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated P| Product Name | HOMEZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox and leucine zipper protein Homez, Homeodomain leucine zipper-containing factor, HOMEZ, KIAA1443 |
| Product description | May function as a transcriptional regulator.1) Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004) |
| Immunogen | This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOMEZ |
|---|---|
| Alternative Names | Homeobox and leucine zipper protein Homez Homeodomain leucine zipper-containing factor HOMEZ KIAA1443 |
| Molecular Weight(MW) | 61 kDa |
Application
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated P| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
