Application
HOXA3 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | HOXA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein Hox-A3, Homeobox protein Hox-1E, HOXA3, HOX1E |
| Product description | In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.1) Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009) |
| Immunogen | This HOXA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human HOXA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOXA3 |
|---|---|
| Alternative Names | Homeobox protein Hox-A3 Homeobox protein Hox-1E HOXA3 HOX1E |
| Molecular Weight(MW) | 46 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
HOXA3 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
