Application
Flow cytometric analysis of 293 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of in mouse kidney tissue lysates (35ug/lane)| Product Name | HOXA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein Hox-A5, Homeobox protein Hox-1C, HOXA5, HOX1C |
| Product description | In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA5 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.1) Chen,Y., Blood 111 (3), 1217-1226 (2008) |
| Immunogen | This HOXA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-223 amino acids from the C-terminal region of human HOXA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | HOXA5 |
|---|---|
| Alternative Names | Homeobox protein Hox-A5 Homeobox protein Hox-1C HOXA5 HOX1C |
| Molecular Weight(MW) | 29 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Drosophila |
Application
Flow cytometric analysis of 293 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of in mouse kidney tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
