Application
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in PC-3 cell line lysates (35ug/lane).| Product Name | HOXC6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein Hox-C6, Homeobox protein CP25, Homeobox protein HHOC8, Homeobox protein Hox-3C, HOXC6, HOX3C |
| Product description | This protein belongs to the homeobox family, members of which encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene, HOXC6, is one of several HOXC genes located in a cluster on chromosome 12. Three genes, HOXC5, HOXC4 and HOXC6, share a 5' non-coding exon.1) Zhang, X., et al. J. Cell. Mol. Med. 11(2):299-306(2007) |
| Immunogen | This HOXC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human HOXC6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOXC6 |
|---|---|
| Alternative Names | Homeobox protein Hox-C6 Homeobox protein CP25 Homeobox protein HHOC8 Homeobox protein Hox-3C HOXC6 HOX3C |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in PC-3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
