Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Ab-78).Application
Western blot analysis of lysed extracts from HeLa cells using HSP27 (Ab-78).| Product Name | HSP27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, CREB2, CREBP1, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27 |
| Product description | Involved in stress resistance and actin organization.1) J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058 |
| Immunogen | HSP27 (Ab-78) antibody was raised against a peptide sequence around aa.76~80 (A-L-S-R-Q) derived from Human HSP27. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names | CMT2F HMN2B HSP27 HSP28 Hsp25 SRP27 HS.76067 HEL-S-102 CREB2 CREBP1 Heat shock 27 kDa protein HSP 27 Stress-responsive protein 27 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of total HSP27 protein. |
| Entrez Gene | 3315 |
|---|---|
| Protein Accession | NP_001531.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Ab-78).
Application
Western blot analysis of lysed extracts from HeLa cells using HSP27 (Ab-78).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
