Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).| Product Name | HSP27 (phospho Ser15) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, ASPL, RCC17, TUG, UBXD9, UBXN9, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25 |
| Product description | Involved in stress resistance and actin organization.1) Tremolada L, et al. (2005) Proteomics. 5 (3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3 (2): 115-124. Benn SC, et al. (2002) Neuron. 36 (1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1 (4): 314-322. |
| Immunogen | HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27. |
| Modification | p-Ser15 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names | CMT2F HMN2B HSP27 HSP28 Hsp25 SRP27 HS.76067 HEL-S-102 ASPL RCC17 TUG UBXD9 UBXN9 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein HSP 27 HSP25 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of HSP27only when phosphorylated at serine 15. |
| Entrez Gene | 3315 |
|---|---|
| Protein Accession | NP_001531.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
