Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).| Product Name | HSP27 (phospho Ser78) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, KIAA0204, STK2, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25 |
| Product description | Involved in stress resistance and actin organization.1) J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058 |
| Immunogen | HSP27 (Phospho-Ser78) antibody was raised against a peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27. |
| Modification | p-Ser78 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names | CMT2F HMN2B HSP27 HSP28 Hsp25 SRP27 HS.76067 HEL-S-102 KIAA0204 STK2 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein HSP 27 HSP25 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of HSP27 only when phosphorylated at serine 78. |
| Entrez Gene | 3315 |
|---|---|
| Protein Accession | NP_001531.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).
Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
