Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser82).Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser82).| Product Name | HSP27 (phospho Ser82) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, CYK8, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25 |
| Product description | Involved in stress resistance and actin organization.1) White MY, et al. (2006) J Mol Cell Cardiol. 40 (6): 761-74Xiankui Sun, et al. (2006) Cell Stress Chaperones. 11 (1): 61–70Matthias Meier; et al. (2001) Hypertension. 38:1260Ali Dana, et al. (2000) Circulation Research. 86: 989 |
| Immunogen | HSP27 (Phospho-Ser82) antibody was raised against a peptide sequence around phosphorylation site of serine 82 (Q-L-S (p) -S-G) derived from Human HSP27. |
| Modification | p-Ser82 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names | CMT2F HMN2B HSP27 HSP28 Hsp25 SRP27 HS.76067 HEL-S-102 CYK8 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein HSP 27 HSP25 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of HSP27 only when phosphorylated at serine 82. |
| Entrez Gene | 3315 |
|---|---|
| Protein Accession | NP_001531.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser82).
Application
Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser82).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
