Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (S78) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HSPB1 using rabbit polyclonal HSPB1 Antibody (S78) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2).| Product Name | HSP27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28 |
| Product description | In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.1) Wano, C., et al., Exp. Cell Res. 298(2):584-592 (2004). |
| Immunogen | This HSP27(HSPB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from human HSP27(HSPB1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names | Heat shock protein beta-1 HspB1 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Heat shock 27 kDa protein HSP 27 Stress-responsive protein 27 SRP27 HSPB1 HSP27 HSP28 |
| Molecular Weight(MW) | 23 kDa |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (S78) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HSPB1 using rabbit polyclonal HSPB1 Antibody (S78) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
