Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP90B (Ab-254).Application
Western blot analysis of lysed extracts from HeLa and A2780 cells using HSP90B (Ab-254).| Product Name | HSP90B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSP84, HSPC2, HSPCB, D6S182, HSP90B, GYS, HSP 84 |
| Product description | Molecular chaperone. Has ATPase activity.1) Ingrid K. Ruf, et, al. (2005) J. Virol ; 79: 14562 - 14569. Christy M. Hebner, et, al. (2006) J. Gen. Virol ; 87: 3183 - 3193. Rika van Huizen, et, al. (2003) J. Biol. Chem ; 278: 15558 - 15564. |
| Immunogen | HSP90B (Ab-254) antibody was raised against a peptide sequence around aa. 252~256 (V-G-S-D-E) derived from Human HSP90B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSP90AB1 |
|---|---|
| Alternative Names | HSP84 HSPC2 HSPCB D6S182 HSP90B GYS HSP 84 |
| Molecular Weight(MW) | 92 kDa |
| Tissue Specificity | This antibody detects endogenous level of total HSP90B protein. |
| Entrez Gene | 3326 |
|---|---|
| Protein Accession | NP_031381.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP90B (Ab-254).
Application
Western blot analysis of lysed extracts from HeLa and A2780 cells using HSP90B (Ab-254).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
