Application
Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (Y525) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).| Product Name | HSPA1A/HSPA1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70 |
| Product description | HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.1) Mueller, T., et al., Transplantation 78(2):292-295 (2004). |
| Immunogen | This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-532 amino acids from human HSPA1A/HSPA1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPA1A |
|---|---|
| Alternative Names | Heat shock 70 kDa protein 1A/1B Heat shock 70 kDa protein 1/2 HSP70-1/HSP70-2 HSP701/HSP702 HSPA1A HSPA1 HSX70 |
| Molecular Weight(MW) | 70 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (Y525) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM281419