Application
Immunohistochemistry staining of HTR3A in heart tissue using HTR3A Antibody.| Product Name | HTR3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTR3A, 5-HT3-A, 5-HT3R-A, 5-HT3 receptor, 5HT3 serotonin receptor, 5-HT3A, 5-HT3a receptor, 5-hydroxytryptamine receptor 3, 5-HT-3, 5-HT3, 5-HT3R, 5HT3R, HTR3, Serotonin receptor 3A |
| Product description | Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord. 5-Ht3a is a member of the 5-HT3 receptor subfamily of ligand gated ion channels, as opposed to all other known 5-HT receptor subtypes that are G protein-coupled receptors. |
| Immunogen | HTR3A antibody was raised against amino acids 21 - 36 of HTR3A (Rat). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| HTR3A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | HTR3A |
|---|---|
| Alternative Names | HTR3A 5-HT3-A 5-HT3R-A 5-HT3 receptor 5HT3 serotonin receptor 5-HT3A 5-HT3a receptor 5-hydroxytryptamine receptor 3 5-HT-3 5-HT3 5-HT3R 5HT3R HTR3 Serotonin receptor 3A |
| Tissue Specificity | In human brain, a 47-50 kD band is observed. |
Application
Immunohistochemistry staining of HTR3A in heart tissue using HTR3A Antibody.| Application Notes | HTR3A antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | HTR3A antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
