The product of HTR3B belongs to the ligand-gated ion channel receptor superfamily. HTR3B encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation.1) Michel, K., et al., (2005) Gastroenterology. 128(5), 1317-26.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HTR3B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
HTR3B antibody can be used for detection of HTR3B by ELISA at 1:312500. HTR3B antibody can be used for detection of HTR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Spleen at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
HTR3B antibody can be used for detection of HTR3B by ELISA at 1:312500. HTR3B antibody can be used for detection of HTR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HTR3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.