Application
Immunohistochemistry staining of Huntingtin in brain cerebellum tissue using Huntingtin Antibody.| Product Name | Huntingtin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HTT, Huntingtin, Huntington disease protein, IT15, HD, HD protein |
| Product description | Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. |
| Immunogen | Huntingtin antibody was raised against amino acids 416-424 from the C-Terminus of Huntingtin (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| Huntingtin antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 1:500, immunofluorescence, and immunoprecipitation.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HTT |
|---|---|
| Alternative Names | HTT Huntingtin Huntington disease protein IT15 HD HD protein |
| Tissue Specificity | pSer421 |
Application
Immunohistochemistry staining of Huntingtin in brain cerebellum tissue using Huntingtin Antibody.| Application Notes | Huntingtin antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 1:500, immunofluorescence, and immunoprecipitation.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store Huntingtin antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
