Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.Application
Antibody used in IHC on Human Breast.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in IHC on Human Liver at 5.0 ug/ml.| Product Name | HYAL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HYAL1, HYAL-1, LUCA1, MGC45987, NAT6 |
| Product description | HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. HYAL1 is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for HYAL1.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.1) Poola, I., (2008) Clin. Cancer Res. 14 (4), 1274-1280. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HYAL1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HYAL1 antibody can be used for detection of HYAL1 by ELISA at 1:62500. HYAL1 antibody can be used for detection of HYAL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HYAL1 |
|---|---|
| Alternative Names | HYAL1 HYAL-1 LUCA1 MGC45987 NAT6 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
Application
Antibody used in IHC on Human Breast.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in IHC on Human Liver at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | HYAL1 antibody can be used for detection of HYAL1 by ELISA at 1:62500. HYAL1 antibody can be used for detection of HYAL1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HYAL1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
