Application
Confocal immunofluorescent analysis of HYOU1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysate from HepG2 cell line, using HYOU1 Antibody at 1:1000 at each lane.| Product Name | HYOU1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170, HYOU1, GRP170, ORP150 |
| Product description | HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.1) Kitao,Y., Matsuyama,T. Antioxid. Redox Signal.(5), 589-595 (2007) |
| Immunogen | This HYOU1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human HYOU1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HYOU1 |
|---|---|
| Alternative Names | Hypoxia up-regulated protein 1 150 kDa oxygen-regulated protein ORP-150 170 kDa glucose-regulated protein GRP-170 HYOU1 GRP170 ORP150 |
| Molecular Weight(MW) | 111 kDa |
Application
Confocal immunofluorescent analysis of HYOU1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HYOU1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysate from HepG2 cell line, using HYOU1 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
