Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | IBSP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IBSP, BSP, BNSP, SP-II, BSP-II |
| Product description | The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.1) Barnes, G.L., et al., (2003) Cancer Res. 63 (10), 2631-2637. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IBSP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IBSP antibody can be used for detection of IBSP by ELISA at 1:62500. IBSP antibody can be used for detection of IBSP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IBSP |
|---|---|
| Alternative Names | IBSP BSP BNSP SP-II BSP-II |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | IBSP antibody can be used for detection of IBSP by ELISA at 1:62500. IBSP antibody can be used for detection of IBSP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IBSP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
