| Product Name | IDO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Indoleamine 2,3-dioxygenase; INDO |
| Product description | IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. |
| Immunogen | Recombinant human IDO. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICCWB |
| ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:200-1:500). Immunocytochemistry: (1:500) . Western Blot: (1:2,000-1:5,000 using ECL suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IDO1 |
|---|---|
| Alternative Names | Indoleamine 2 3-dioxygenase INDO |
| Tissue Specificity | Recognizes human IDO. Detects a band of ~45kDa by Western blot. |
| Entrez Gene | 3620 |
|---|---|
| Protein Accession | NP_002155.1 |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:10,000). Flow Cytometry: (1:200-1:500). Immunocytochemistry: (1:500) . Western Blot: (1:2,000-1:5,000 using ECL suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
