Application
Immunohistochemistry of IFIT1 in human small intestine tissue with IFIT1 antibody at 5 μg/mL.Application
Immunofluorescence of IFIT1 in human small intestine tissue with IFIT1 antibody at 20 μg/mL.Application
Western blot analysis of IFIT1 in rat small intestine tissue lysate with IFIT1 antibody at 1 μg/ml.| Product Name | IFIT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFIT1 Antibody: C56, P56, G10P1, IFI56, ISG56, IFI-56, IFIT-1, IFNAI1, RNM561, IFI-56K, Interferon-induced protein with tetratricopeptide repeats 1, Interferon-induced 56 kDa protein |
| Product description | The interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) protein is a member of a family of tetratricopeptide repeat-containing proteins whose transcription is upregulated by interferons, virus infection, and molecular patterns such as dsRNA or lipopolysaccharides (1,2). These proteins have been suggested to induce anti-viral cellular activities in response to infection (2). Together with the interferon-induced transmembrane protein 1 (IFITM1), IFIT1 inhibits the replication of the Hepatitis C virus, suggesting that it may be useful in the development of therapeutic treatments (3).1) Wathelet M, Moutschen S, Defilippi P, et al. Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferons. Eur. J. Biochem. 1986; 155:11-7. |
| Immunogen | IFIT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IFIT1. The immunogen is located within amino acids 290 - 340 of IFIT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| IFIT1 antibody can be used for detection of IFIT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIT1 |
|---|---|
| Alternative Names | IFIT1 Antibody: C56 P56 G10P1 IFI56 ISG56 IFI-56 IFIT-1 IFNAI1 RNM561 IFI-56K Interferon-induced protein with tetratricopeptide repeats 1 Interferon-induced 56 kDa protein |
| Molecular Weight(MW) | Predicted: 53 kDa Observed: 53 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | IFIT1 antibody is human, mouse and rat reactive. At least three isoforms of IFIT1 are known to exist. This antibody is predicted to not cross-react with other members of the IFIT protein family. |
Application
Immunohistochemistry of IFIT1 in human small intestine tissue with IFIT1 antibody at 5 μg/mL.
Application
Immunofluorescence of IFIT1 in human small intestine tissue with IFIT1 antibody at 20 μg/mL.
Application
Western blot analysis of IFIT1 in rat small intestine tissue lysate with IFIT1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | IFIT1 antibody can be used for detection of IFIT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IFIT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IFIT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
