IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.1) Hertel, J.K., (2008) Diabetologia 51 (6), 971-977.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IGFBP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
IGFBP2 antibody can be used for detection of IGFBP2 by ELISA at 1:12500. IGFBP2 antibody can be used for detection of IGFBP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
IGFBP2 antibody can be used for detection of IGFBP2 by ELISA at 1:12500. IGFBP2 antibody can be used for detection of IGFBP2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IGFBP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.