Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-ε (Phospho-Ser22).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).| Product Name | IkB epsilon (phospho Ser22) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKBE, BHLHE39, I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon |
| Product description | Inhibits NF-κ-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-κ-B p50-p65 and p50-c-Rel complexes.1) Shirane M, et al. (1999) J Biol Chem; 274 (40): 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z. J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488. |
| Immunogen | IkB-epsilon (Phospho-Ser22) antibody was raised against a peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Human IkB-ε. |
| Modification | p-Ser22 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIE |
|---|---|
| Alternative Names | IKBE BHLHE39 I-kappa-B-epsilon IKBE IkappaBepsilon NF-kappa-BIE NF-kappaB inhibitor epsilon |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | This antibody detects endogenous level of IkappaB-ε only when phosphorylated at serine 22. |
| Entrez Gene | 4794 |
|---|---|
| Protein Accession | NP_004547.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-ε (Phospho-Ser22).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
