Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Ab-23).Application
Western blot analysis of lysed extracts from HeLa, 293 and 3T3 cells using IKK α (Ab-23).| Product Name | IKK a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, H2BFF, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1 |
| Product description | Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ-B-regulated promoters during inflammatory responses triggered by cytokines.1) Yuan ZQ, et al. (2002) J Biol Chem; 277 (33): 29973-82. Ozes ON, et al. (1999) Nature; 401 (6748): 82-5. |
| Immunogen | IKK a (Ab-23) antibody was raised against a peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK α. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHUK |
|---|---|
| Alternative Names | IKK1 IKKA IKBKA TCF16 NFKBIKA IKK-alpha H2BFF I kappa-B kinase alpha I-kappa-B kinase 1 IKK-A IKK1 |
| Molecular Weight(MW) | 85 kDa |
| Tissue Specificity | This antibody detects endogenous level of total IKKα protein. |
| Entrez Gene | 1147 |
|---|---|
| Protein Accession | NP_001269.3 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Ab-23).
Application
Western blot analysis of lysed extracts from HeLa, 293 and 3T3 cells using IKK α (Ab-23).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
