Application
Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 μg/mL.Application
Immunofluorescence of IKKa in HeLa cells with IKKa antibody at 2 μg/mL.Application
Western blot analysis of IKK alpha in HeLa cell lysate with IKK alpha antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | IKK alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha |
| Product description | IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-alpha. IKKα is expressed in a variety of human tissues.1) DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54 |
| Immunogen | IKK alpha antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK alpha. The immunogen is located within the last 50 amino acids of IKK alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IKK alpha antibody can be used for detection of IKK alpha by Western blot 0.5 μg/mL. A 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHUK |
|---|---|
| Alternative Names | IKK alpha Antibody: IKK1 IKKA IKBKA TCF16 NFKBIKA IKK-alpha Inhibitor of nuclear factor kappa-B kinase subunit alpha Conserved helix-loop-helix ubiquitous kinase I-kappa-B kinase alpha |
| Molecular Weight(MW) | 85 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | This polyclonal antibody has no cross response to IKKb or IKKy. |
Application
Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 μg/mL.
Application
Immunofluorescence of IKKa in HeLa cells with IKKa antibody at 2 μg/mL.
Application
Western blot analysis of IKK alpha in HeLa cell lysate with IKK alpha antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IKK alpha antibody can be used for detection of IKK alpha by Western blot 0.5 μg/mL. A 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IKK alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
