Application
Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | IKK- alpha/ beta (phospho Ser176/177) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ40509, I-kappa-B kinase, IKBKB, kinase beta, NFKBIKB |
| Product description | Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.1) Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98 |
| Immunogen | IKK- alpha/ beta (phospho Ser176/177) antibody was raised against a peptide sequence around phosphorylation site of serine 176/177 (Q-G-S (p)-L-C) derived from Human IKK-alpha/beta. |
| Modification | p-Ser176/177 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHUK |
|---|---|
| Alternative Names | FLJ40509 I-kappa-B kinase IKBKB kinase beta NFKBIKB |
| Molecular Weight(MW) | 85 kDa |
| Tissue Specificity | The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177. |
| Entrez Gene | 1147 |
|---|---|
| Protein Accession | NP_001269.3 |
Application
Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
