Application
Immunofluorescence of IKZF2 in human spleen tissue with IKZF2 antibody at 20 μg/mL.Application
Immunohistochemistry of IKZF2 in human spleen tissue with IKZF2 antibody at 5 μg/mL.Application
Western blot analysis of IKZF2 in EL4 cell lysate with IKZF2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | IKZF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ikaros family zinc finger 2 (Helios), ANF1A2, HELIOS, ZNF1A2, ZNFN1A2 |
| Product description | The Ikaros family zinc finger 2 (IKZF2) is a member of the Ikaros family of zinc-finger proteins, a family of hematopoietic-specific transcription factors involved in the regulation of lymphocyte development (1,2). IKZF2 forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development (3). Its expression is a marker for T cell activation and proliferation (4).1) Hahm K, Cobb BS, McCarty AS, et al. Helios, a T cell-restricted Ikaros family member that quantitatively associates with Ikaros at centromeric heterochromatin. Genes Dev. 1998; 12:782-96. |
| Immunogen | IKZF2 antibody was raised against a 19 amino acid peptide near the amino terminus of human IKZF2. The immunogen is located within amino acids 50 - 100 of IKZF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IKZF2 antibody can be used for detection of IKZF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKZF2 |
|---|---|
| Alternative Names | Ikaros family zinc finger 2 (Helios) ANF1A2 HELIOS ZNF1A2 ZNFN1A2 |
| Molecular Weight(MW) | Predicted: 58 kDa Observed: 60 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | IKZF2 antibody is human, mouse and rat reactive. At least two isoforms of IKZF2 are known to exist; this antibody will detect both isoforms. IKZF2 antibody is predicted to not cross-react with other IKZF proteins. |
Application
Immunofluorescence of IKZF2 in human spleen tissue with IKZF2 antibody at 20 μg/mL.
Application
Immunohistochemistry of IKZF2 in human spleen tissue with IKZF2 antibody at 5 μg/mL.
Application
Western blot analysis of IKZF2 in EL4 cell lysate with IKZF2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | IKZF2 antibody can be used for detection of IKZF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IKZF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IKZF2 antibody is supplied in PBS containing 0.02% sodium azide. |
