Application
Antibody used in IHC on Human Liver.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | IKZF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKZF3, AIO, AIOLOS, ZNFN1A3, RP11-94L15.2 |
| Product description | IKZF3 is a member of the Ikaros family of zinc-finger proteins. It is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoetic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.1) Koipally, J. (2002) J. Biol. Chem. 277 (31), 27697-27705. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IKZF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| IKZF3 antibody can be used for detection of IKZF3 by ELISA at 1:312500. IKZF3 antibody can be used for detection of IKZF3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IKZF3 |
|---|---|
| Alternative Names | IKZF3 AIO AIOLOS ZNFN1A3 RP11-94L15.2 |
| Molecular Weight(MW) | 52 kDa, 58 kDa, 53 kDa, 54 kDa, 47 kDa, 49 kDa |
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | IKZF3 antibody can be used for detection of IKZF3 by ELISA at 1:312500. IKZF3 antibody can be used for detection of IKZF3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IKZF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
