Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.| Product Name | IL-15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD122, IL15RB, P70-75Interleukin-15IL-15 |
| Product description | IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-15 (human Interleukin-15). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-15 (2.0 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required. ELISA: To detect hIL-15 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-15. To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL2RB |
|---|---|
| Alternative Names | CD122 IL15RB P70-75Interleukin-15IL-15 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-15 (2.0 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required. ELISA: To detect hIL-15 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-15. To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-15 (XP-5169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-15 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
