Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas. The recommended concentration is 2.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.Application
To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-17D by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-17D (38-277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D.| Product Name | IL-17D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL27, IL-27, IL-17D, IL27, UNQ3096/PRO21175, Interleukin-17D, Interleukin-27 |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human IL-17D. Human IL-17D specific antibody was purified by affinity chromatography employing an immobilized Human IL-17D matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect Human IL-17D by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-17D (38-277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D. Western Blot: To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IL17D |
|---|---|
| Alternative Names | IL27 IL-27 IL-17D IL27 UNQ3096/PRO21175 Interleukin-17D Interleukin-27 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas. The recommended concentration is 2.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
Application
To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-17D by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-17D (38-277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D.| Application Notes | ELISA: Sandwich: To detect Human IL-17D by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-17D (38-277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D. Western Blot: To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
