Application
Western blot analysis of IL-21 expression in HL-60 cell lysate in (A) the absence or (B) the presence of blocking peptide with IL-21 antibody at 1 μg /ml.Application
Immunocytochemistry of IL-21 in K562 cells with IL-21 antibody at 2 μg/mL.| Product Name | IL-21 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IL-21 Antibody: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.1) Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 2000; 408:57-63. |
| Immunogen | IL-21 antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC,WB |
| IL-21 antibody can be used for detection of IL-21 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL21 |
|---|---|
| Alternative Names | IL-21 Antibody: Za11 IL-21 Interleukin-21 Za11 |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 18 kDa |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family. |
Application
Western blot analysis of IL-21 expression in HL-60 cell lysate in (A) the absence or (B) the presence of blocking peptide with IL-21 antibody at 1 μg /ml.
Application
Immunocytochemistry of IL-21 in K562 cells with IL-21 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | IL-21 antibody can be used for detection of IL-21 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-21 Antibody is supplied in PBS containing 0.02% sodium azide. |
