Application
Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 μg/mL.Application
Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 μg/mL.Application
Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | IL-22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-22 Antibody: TIFa, IL-21, IL-22, ILTIF, IL-TIF, IL-D110, zcyto18, TIFIL-23, ZCYTO18, UNQ3099/PRO10096, Interleukin-22, Cytokine Zcyto18 |
| Product description | Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).1) Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J. Biol. Chem. 2000; 275:31335-9. |
| Immunogen | IL-22 antibody was raised against a 17 amino acid peptide near the center of human IL-22 . The immunogen is located within amino acids 90 - 140 of IL-22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL22 |
|---|---|
| Alternative Names | IL-22 Antibody: TIFa IL-21 IL-22 ILTIF IL-TIF IL-D110 zcyto18 TIFIL-23 ZCYTO18 UNQ3099/PRO10096 Interleukin-22 Cytokine Zcyto18 |
| Molecular Weight(MW) | Predicted: 20 kDa Observed: 17 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist. |
Application
Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 μg/mL.
Application
Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 μg/mL.
Application
Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-22 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-22 antibody is supplied in PBS containing 0.02% sodium azide. |
