Application
Immunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 μg/mL.Application
Immunofluorescence of IL-22R in rat kidney tissue with IL-22R antibody at 20 μg/ml.Application
Immunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.Application
Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.Application
Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg /ml.| Product Name | IL-22 Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-22 Receptor Antibody: IL22R, CRF2-9, IL22R1, IL22R, Interleukin-22 receptor subunit alpha-1, Cytokine receptor class-II member 9, IL-22 receptor subunit alpha-1 |
| Product description | IL-22 Receptor Antibody: A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.1) Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9. |
| Immunogen | IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor. The immunogen is located within the first 50 amino acids of IL-22 Receptor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL22RA1 |
|---|---|
| Alternative Names | IL-22 Receptor Antibody: IL22R CRF2-9 IL22R1 IL22R Interleukin-22 receptor subunit alpha-1 Cytokine receptor class-II member 9 IL-22 receptor subunit alpha-1 |
| Molecular Weight(MW) | Predicted: 63 kDa Observed: 65 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Immunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 μg/mL.
Application
Immunofluorescence of IL-22R in rat kidney tissue with IL-22R antibody at 20 μg/ml.
Application
Immunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Application
Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.
Application
Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg /ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-22 Receptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-22 Receptor Antibody is supplied in PBS containing 0.02% sodium azide. |
