Application
Immunohistochemistry of IL-23 in mouse pancreas tissue with IL-23 antibody at 2 μg/mL.Application
Immunofluorescence of IL-23 in Mouse Pancreas tissue with IL-23 antibody at 20 μg/mL.Application
Western blot analysis of IL-23 in mouse pancreas tissue lysate with IL-23 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IL-23 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha |
| Product description | IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.1) Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31. |
| Immunogen | IL-23 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL23A |
|---|---|
| Alternative Names | IL-23 Antibody: P19 SGRF IL-23 IL-23A IL23P19 UNQ2498/PRO5798 Interleukin-23 subunit alpha Interleukin-23 subunit p19 IL-23 subunit alpha |
| Molecular Weight(MW) | Predicted: 21 kDa Observed: 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Horse: (94%) |
Application
Immunohistochemistry of IL-23 in mouse pancreas tissue with IL-23 antibody at 2 μg/mL.
Application
Immunofluorescence of IL-23 in Mouse Pancreas tissue with IL-23 antibody at 20 μg/mL.
Application
Western blot analysis of IL-23 in mouse pancreas tissue lysate with IL-23 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1414 - Mouse Pancreas Tissue Lysate |
|---|---|
| Application Notes | IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-23 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-23 Antibody is supplied in PBS containing 0.02% sodium azide. |
