Application
Western blot analysis of 125 ng of recombinant human IL-34 with IL-34 antibody at 1 μg/ml.Application
Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | IL-34 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-34 Antibody: IL-34, C16orf77, Interleukin-34, IL-34 |
| Product description | IL-34 Antibody: Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.1) Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science2008; 320:807-11. |
| Immunogen | IL-34 antibody was raised against a 13 amino acid synthetic peptide from near the center of human IL-34. The immunogen is located within amino acids 60 - 110 of IL-34. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL34 |
|---|---|
| Alternative Names | IL-34 Antibody: IL-34 C16orf77 Interleukin-34 IL-34 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (79%) |
Application
Western blot analysis of 125 ng of recombinant human IL-34 with IL-34 antibody at 1 μg/ml.
Application
Western blot analysis of IL-34 in human brain tissue lysate with IL-34 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-34 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-34 Antibody is supplied in PBS containing 0.02% sodium azide. |
