Application
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-9.| Product Name | IL-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P40, HP40, IL-9Interleukin-9, Cytokine P40, IL-9 |
| Product description | IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors; but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-9 (human Interleukin-9). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-9 (0.6 ng/mL), a concentration of 0.15 - 0.025 μg/mL of this antibody is required. ELISA: To detect hIL-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-9. Sandwich: To detect hIL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL9 |
|---|---|
| Alternative Names | P40 HP40 IL-9Interleukin-9 Cytokine P40 IL-9 |
Application
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-9 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-9.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-9 (0.6 ng/mL), a concentration of 0.15 - 0.025 μg/mL of this antibody is required. ELISA: To detect hIL-9 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-9. Sandwich: To detect hIL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-9 (XP-5200Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-9. Western Blot: To detect hIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-9 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
