Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Product Name | IL1R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, IL-1R-alpha, Interleukin-1 receptor type I, p80, CD121a, Interleukin-1 receptor type 1, membrane form, mIL-1R1, mIL-1RI, Interleuki |
| Product description | IL1R is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. IL1R is a receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. The gene for this protein and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.1) Debets, R., et al., J. Immunol. 167(3):1440-1446 (2001). |
| Immunogen | This IL1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human IL1R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL1R1 |
|---|---|
| Alternative Names | Interleukin-1 receptor type 1 IL-1R-1 IL-1RT-1 IL-1RT1 CD121 antigen-like family member A Interleukin-1 receptor alpha IL-1R-alpha Interleukin-1 receptor type I p80 CD121a Interleukin-1 receptor type 1 membrane form mIL-1R1 mIL-1RI Interleuki |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
