Application
Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody at 1:1000 at each lane.Application
Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | IL4R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Soluble interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protei |
| Product description | Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.1) Idzerda R.L.,et al.J. Exp. Med. 171:861-873(1990). |
| Immunogen | This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL4R |
|---|---|
| Alternative Names | Interleukin-4 receptor subunit alpha IL-4 receptor subunit alpha IL-4R subunit alpha IL-4R-alpha IL-4RA CD124 Soluble interleukin-4 receptor subunit alpha Soluble IL-4 receptor subunit alpha Soluble IL-4R-alpha sIL4Ralpha/prot IL-4-binding protei |
| Molecular Weight(MW) | 90 kDa |
Application
Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody at 1:1000 at each lane.
Application
Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
