Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Product Name | ILF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILF3, CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, TCP110, MPHOSPH4, NF-AT-90 |
| Product description | ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.1) Nie, Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be used for detection of ILF3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ILF3 |
|---|---|
| Alternative Names | ILF3 CBTF DRBF MMP4 MPP4 NF90 NFAR NF110 NF90a NF90b NFAR2 TCP80 DRBP76 NF110b NFAR-1 TCP110 MPHOSPH4 NF-AT-90 |
| Molecular Weight(MW) | 76 kDa, 95 kDa, 75 kDa |
Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be used for detection of ILF3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ILF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
