Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of IMPDH2 Antibody in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane)Application
IMPDH2 Antibody IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | IMPDH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inosine-5'-monophosphate dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMP dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPD 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPDH 2 {ECO:0000255|HAMAP-Rule:MF_03156}, 111205 {ECO:0000255|HAMAP-Rule:MF_03156} |
| Product description | IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. Its gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.1) Sombogaard,F., Pharmacogenet. Genomics 19 (8), 626-634 (2009) |
| Immunogen | This IMPDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-210 amino acids from the Central region of human IMPDH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | IMPDH2 |
|---|---|
| Alternative Names | Inosine-5'-monophosphate dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156} IMP dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156} IMPD 2 {ECO:0000255|HAMAP-Rule:MF_03156} IMPDH 2 {ECO:0000255|HAMAP-Rule:MF_03156} 111205 {ECO:0000255|HAMAP-Rule:MF_03156} |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of IMPDH2 Antibody in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane)
Application
IMPDH2 Antibody IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
