Application
Western blot analysis of INPP5D using rabbit polyclonal INPP5D Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INPP5D gene (Lane 2).| Product Name | INPP5D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 3,5-trisphosphate 5-phosphatase 1, Inositol polyphosphate-5-phosphatase of 145 kDa, SIP-145, SH2 domain-containing inositol 5'-phosphatase 1, SH2 domain-containing inositol phosphatase 1, SHIP-1, p150Ship, hp51CN, INPP5D, SHIP, SHIP1 |
| Product description | INPP5D is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and it is a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeliod cell proliferation and survival.1) Gilby,D.C., Leukemia 21 (11), 2390-2393 (2007) |
| Immunogen | This INPP5D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the Central region of human INPP5D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | INPP5D |
|---|---|
| Alternative Names | Phosphatidylinositol 3 5-trisphosphate 5-phosphatase 1 Inositol polyphosphate-5-phosphatase of 145 kDa SIP-145 SH2 domain-containing inositol 5'-phosphatase 1 SH2 domain-containing inositol phosphatase 1 SHIP-1 p150Ship hp51CN INPP5D SHIP SHIP1 |
| Molecular Weight(MW) | 133 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of INPP5D using rabbit polyclonal INPP5D Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INPP5D gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
