Application
Western blot analysis of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 μg/mL.| Product Name | Integrin alpha 4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D |
| Product description | Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.1) Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol.2008; 25:376-87. |
| Immunogen | Integrin alpha 4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4. The immunogen is located within amino acids 220 - 270 of Integrin alpha 4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGA4 |
|---|---|
| Alternative Names | Integrin alpha 4 Antibody: IA4 CD49D Integrin alpha-4 CD49 antigen-like family member D |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it. |
Application
Western blot analysis of Integrin alpha 4 in rat spleen tissue lysate with Integrin alpha 4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of Integrin alpha 4 in Jurkat cells with Integrin alpha 4 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Integrin alpha 4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide. |
