Application
Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).| Product Name | Interferon-a Receptor (phospho Ser535 / 539) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AVP, IFRC, IFNAR, IFNBR, IFN-alpha-REC, Cytokine receptor class-II member 1, IFN-R-1 |
| Product description | Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1.1) Suresh Kumar, K.G., Tang, W., Ravindranath, A.K., Clark, W.A., Croze, E., Fuchs, S.Y., "SCFHOS ubiquitin ligase mediates the ligand-induced down-regulation of the interferon- receptor," The EMBO Journal, 22 (2003), 5480-5490. |
| Immunogen | IFNAR1 (Ser535/539) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the human Phospho-Ser535, 539 Type I Interferon-a Receptor Subunit 1 (IFNAR1). |
| Modification | p-Ser535 / 539 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFNAR1 |
|---|---|
| Alternative Names | AVP IFRC IFNAR IFNBR IFN-alpha-REC Cytokine receptor class-II member 1 IFN-R-1 |
| Molecular Weight(MW) | 110-130 |
| Tissue Specificity | Interferon a Receptor antibody is specific for IFNAR1 phosphorylated at Ser535,539. |
Application
Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).| Application Notes | Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
