Application
Immunohistochemistry staining of IRAK4 in kidney tissue using IRAK4 Antibody.| Product Name | IRAK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members. The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-κB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified. IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5). IRAK4, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK4 has auto- and cross-phosphorylation kinase activity and has been shown to phosphorylate and activate IRAK1. Additionally, IRAK4 interacts with IRAK1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK4 can activate NF-kappaB as well as mitogen-activated protein (MAP) kinase pathways. IRAK4 (-/-) mice are resistant to a lethal dose of lipopolysaccharide and are severely impaired in their responses to viral and bacterial challenges. At least two mRNA transcripts have been reported: 3.0- and 4.4-kb. IRAK4, also referrred to as NY-REN-64, was first identified as an anitgen in a screen of renal tumors. |
| Immunogen | IRAK4 antibody was raised against amino acids 38 - 54 of IRAK4 (Mouse). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IRAK4 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 10 - 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | IRAK4 |
|---|---|
| Alternative Names | IRAK4 IPD1 IRAK-4 NY-REN-64 REN64 |
Application
Immunohistochemistry staining of IRAK4 in kidney tissue using IRAK4 Antibody.| Application Notes | IRAK4 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 10 - 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK4 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
Data sheet for OM282285