Application
Immunocytochemistry of IRAK-4 in K562 cells with IRAK-4 antibody at 10 μg/mL.Application
Immunofluorescence of IRAK-4 in K562 cells with IRAK-4 antibody at 10 μg/mL.Application
Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) μg/mL, respectively.| Product Name | IRAK-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRAK-4 Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-κB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.1) Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31. |
| Immunogen | IRAK-4 antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4. The immunogen is located within the last 50 amino acids of IRAK-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAK4 |
|---|---|
| Alternative Names | IRAK-4 Antibody: IPD1 REN64 IRAK-4 NY-REN-64 Interleukin-1 receptor-associated kinase 4 Renal carcinoma antigen NY-REN-64 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 51 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family. |
Application
Immunocytochemistry of IRAK-4 in K562 cells with IRAK-4 antibody at 10 μg/mL.
Application
Immunofluorescence of IRAK-4 in K562 cells with IRAK-4 antibody at 10 μg/mL.
Application
Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) μg/mL, respectively.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRAK-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide. |
