Application
Immunocytochemistry of IRF2BP2 in A20 cells with IRF2BP2 antibody at 2.5 μg/mL.Application
Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | IRF2BP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | IRF2BP2 Antibody: IRF2BP2, like the related protein IRF2BP1, is a co-repressor that interacts specifically with the C-terminal repression domain of Interferon Regulatory Factor 2 (IRF2). IRF2BP2 is a direct target gene of p53 and is involved in cell survival during the p53 stress response, able to impede the p53-mediated transactivation of p21 and Bax. IRF2BP2 is also a co-factor of VGLL4 and is required to induce the expression of vascular endothelial growth factor A (VEGF-A) in muscle. It is normally found in the nucleus of skeletal muscle and cardiac cells, but can be found in the cytoplasm during skeletal muscle differentiation.1) Childs KS and Goodbourn S. Identification of novel co-repressor molecules for interferon regulatory factor-2. Nuc. Acids Res. 2003; 31:3016-26 |
| Immunogen | IRF2BP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human IRF2BP2. The immunogen is located within amino acids 440 - 490 of IRF2BP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| IRF2BP2 antibody can be used for detection of IRF2BP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF2BP2 |
|---|---|
| Alternative Names | IRF2BP2 Antibody: Interferon regulatory factor 2-binding protein 2 IRF-2-binding protein 2 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | At least two isoforms of IRF2BP2 are known to exist; this antibody will detect both. This antibody is predicted to not cross-react with any FOXD4 protein family members. |
Application
Immunocytochemistry of IRF2BP2 in A20 cells with IRF2BP2 antibody at 2.5 μg/mL.
Application
Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | IRF2BP2 antibody can be used for detection of IRF2BP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | IRF2BP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IRF2BP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
