Application
Antibody used in WB on Human Lymphnode at 0.15 ug/ml.| Product Name | IRF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The activation of Toll-like receptors (TLRs) is central to innate and adaptive immunity. All TLRs use the adaptor MyD88 for signaling. IRF-5 is generally involved downstream of the TLR-MyD88 signalling pathway for gene induction of proinflammatory cytokines, such as interleukin-6 (IL-6), IL-12 and tumour-necrosis factor-alpha.1) Takaoka, A., et al., (2005) Nature 434 (7030), 243-249. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse IRF5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IRF5 antibody can be used for detection of IRF5 by ELISA at 1:62500. IRF5 antibody can be used for detection of IRF5 by western blot at 0.15 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRF5 |
|---|---|
| Alternative Names | IRF5 mirf5 AW491843 |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on Human Lymphnode at 0.15 ug/ml.| Positive Control | 1) Cat. No. 1369 - Human Lymph node Tissue Lysate |
|---|---|
| Application Notes | IRF5 antibody can be used for detection of IRF5 by ELISA at 1:62500. IRF5 antibody can be used for detection of IRF5 by western blot at 0.15 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
