Application
Western blot analysis of anti-IRF8 Antibody in HL60 cell line lysates (35ug/lane)Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IRF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.1) McGough,J.M., Mol. Cancer Res.(12), 1841-1851 (2008) |
| Immunogen | This IRF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-409 amino acids from the C-terminal region of human IRF8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | IRF8 |
|---|---|
| Alternative Names | Interferon regulatory factor 8 IRF-8 Interferon consensus sequence-binding protein H-ICSBP ICSBP IRF8 ICSBP1 |
| Molecular Weight(MW) | 48 kDa |
Application
Western blot analysis of anti-IRF8 Antibody in HL60 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
