IRX3 is a member of the Iroquois homeobox gene family that encodes a protein known for its essential role in spinal cord development.1) Sun, T., et al., (2006) Dev. Biol. 292 (1), 152-164.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IRX3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
IRX3 antibody can be used for detection of IRX3 by ELISA at 1:1562500. IRX3 antibody can be used for detection of IRX3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse NIH-3T3 at 5.0 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
IRX3 antibody can be used for detection of IRX3 by ELISA at 1:1562500. IRX3 antibody can be used for detection of IRX3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRX3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt