IRX6 is one Iroquois (Irx) proteins comprise a family of homeodomain-containing transcription factors involved in patterning and regionalization of embryonic tissues in both vertebrates and invertebrates.1) Pan, L., et al., (2005) Development 132 (4), 703-712.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IRX6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
IRX6 antibody can be used for detection of IRX6 by ELISA at 1:12500. IRX6 antibody can be used for detection of IRX6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
IRX6 antibody can be used for detection of IRX6 by ELISA at 1:12500. IRX6 antibody can be used for detection of IRX6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRX6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt